Assess Transcriptionally Active HPV Biomarkers in Head and Neck Cancer Biopsies

Detection of Gold-standard E6/E7 Oncogene Transcripts Using RNAscope^® ISH

Evidence for transcriptional active of the viral oncogenes E6/E7 is regarded as the gold standard for presence of clinically relevant high-risk human papillomavirus (HPV), but detection of E6/E7 mRNA can be challenging using conventional techniques (Bishop et al., 2013). As a causal agent in head and neck squamous cell carcinoma (HNSCC), it is critical that the detection method enable pathologist review of tissue morphology and be of the highest specificity and sensitivity for accurate assessment of within the tissue microenvironment of FFPE specimens (Figure 1, 2). RNAscope HPV Biomarker Detection Reagents and its proprietary “double Z” oligonucleotide probes specific for each subtype E6/E7 mRNA enable high specificity detection of viral transcripts in routine FFPE tumor biopsies.