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The Pathologist / Issues / 2025 / Aug / Introducing Mass Spectrometry Histochemistry
Histology Career Pathways Professional Development Analytical science Microscopy and imaging Technology and innovation Histology Molecular Pathology

Introducing Mass Spectrometry Histochemistry

Pathology’s future lies in unlocking the molecular signals preserved in FFPE tissue

By Peter Verhaert 08/07/2025 Discussion 6 min read
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5 Key Takeaways
  • 1

    Mass spectrometry imaging (MSI) has struggled to gain adoption in clinical diagnostics despite its analytical power and potential.

  • 2

    Traditional MSI relies on fresh-frozen samples, while most pathology labs use formalin-fixed paraffin-embedded (FFPE) tissues.

  • 3

    Recent research shows high-resolution mass spectrometry histochemistry (MSHC) can effectively analyze archived FFPE tissues.

  • 4

    MSHC can enhance diagnostic accuracy and integrate into existing lab workflows without altering specimen processing.

  • 5

    The implementation of MSHC could revolutionize disease classification and treatment personalization in pathology.

This content is an AI-generated, fully rewritten summary based on a published scholarly article. It does not reproduce the original text and is not a substitute for the original publication. Readers are encouraged to consult the source for full context, data, and methodology.

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References

  1. MRL Paine et al., Anal Chem, 90, 15 (2018). PMID: 29975508.
  2. G Frache et al., Methods Mol Biol (2025). PMID: 40498187.
  3. YL Cintron-Diaz et al., J Am Soc Mass Spectrom, 33, 4 (2022). PMID: 35258288.
  4. P Verhaert et al., “High Resolution Mass Spectrometry of Cystine-Containing Neuropeptides in Histological Sections of Human FFPE Tissue Banks,” in Cysteine – New Insights. IntechOpen (2024). DOI: 10.5772/intechopen.1004948.

About the Author(s)

Peter Verhaert

Founder of ProteoFormiX, Special Lecturer Translational Cell & Tissue Research Unit at the University of Leuven Faculty of Medicine, Belgium, and Visiting Professor at the University of Witwatersrand, South Africa

More Articles by Peter Verhaert

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