Objective:

To explore the potential of mass spectrometry histochemistry (MSHC) in clinical pathology, particularly using archived formalin-fixed paraffin-embedded (FFPE) tissues.

Approach:

• Research Background: Mass spectrometry imaging (MSI) has struggled for adoption in clinical diagnostics due to reliance on fresh-frozen samples, while FFPE tissues are the standard in pathology.
• Methodology: Recent research demonstrates effective high-resolution MSI on archived FFPE tissues, utilizing optimized protocols and advanced instrumentation.
• Implementation Phases: Proposes a three-phased rollout: retrospective disease typing and biomarker discovery, validation for clinical use, and predictive and prognostic testing.

Key Findings:

• MSHC can effectively analyze small and labile compounds from archived FFPE tissues.
• It offers improved molecular specificity and spatial resolution compared to traditional methods.
• MSHC can be integrated into existing lab workflows without altering specimen processing.

Interpretation:

Limitations:

• The need for collaborative engagement from the pathology community for case selection and validation.
• Potential challenges in integrating MSHC into routine diagnostics.

Conclusion:

Sources:

• Peter Verhaert