3 Topic Commentaries

Aptamer Tests Face the Validation Challenge

Aptamer Tests Face the Validation Challenge

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  Andrew N. Hoofnagle, MD, PhD

  Clinical Chemistry / Laboratory Medicine

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  University of Washington, Department of Laboratory Medicine & Pathology

  More concretely, the SomaLogic platform has good precision for almost all of its assays, but what if the platform is precisely measuring noise? Indeed, the influence of related proteins and matrix components has never been properly documented.

  Source
• 

  Manuel Mayr, MD, PhD

  Cardiovascular Proteomics

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  King’s College London, British Heart Foundation Centre, School of Cardiovascular Medicine and Sciences

  Proof of principle, however, still requires independent validation of protein levels through orthogonal validation techniques and linkage to cis-acting Mendelian randomization that do not have epitope effects. In particular, changes in electric charge caused by amino acid substitution may alter the binding properties of the negatively charged aptamers.

  Source
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  John S. Tsang, PhD

  Systems Immunology / Proteomics Bioinformatics

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  National Institute of Allergy and Infectious Diseases, National Institutes of Health

  Firstly, we assess different normalization procedures and point out caveats in which a departure from standard strategies is needed to avoid increasing plate effects, while illustrating these observations with data from a multiplate longitudinal vaccination study.

  Source