5 Key Takeaways

• 1

  Complex in vitro models (CIVMs) like blood-brain barrier organoids enhance preclinical toxicity testing by simulating human tissue structures.

• 2

  Standard histological techniques are essential for validating organoids, bridging bioengineering with established safety evaluation practices.

• 3

  AI algorithms can automate single-cell toxicity assessments, improving efficiency and precision in evaluating organoid models.

• 4

  Multidisciplinary collaboration among biologists, engineers, and pathologists is crucial for developing reliable organoid-based pathology workflows.

• 5

  Future advancements in 3D modeling and automation will enhance the diagnostic utility of organoid-based pathology in personalized medicine.