Development of a predictive spatial signature in human tumors using Multiplexed Ion Beam Imaging (MIBI)
sponsored by IONPATH
February 24th, 2022, at 10:00am PST
Understanding the role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires tools that can rapidly and consistently quantify the expression of multiple proteins while preserving spatial information. To meet this need, we deployed Multiplexed Ion Beam Imaging (MIBI) to visualize up to 40 metal labeled antibodies at subcellular resolution.
Published recently in Cell, we used MIBI to examine ductal carcinoma in situ (DCIS)—a pre-invasive lesion thought to be a precursor to invasive breast cancer (IBC) revealing coordinated TME transitions delineated based on the location and function of myoepithelium, fibroblasts, and immune cells. This Breast PreCancer Atlas study offers new insight into drivers of IBC relapse and emphasizes the importance of the TME in regulating these processes.
Learning Objectives:
- Understand the role of single-cell function with tissue structure context when researching the tumor microenvironment (TME) and other tissue disease states
- Review the requirements for high-multiplex imaging when developing and implementing actionable spatial signatures to predict disease progression or patient response
- Learn how multiplexed ion beam imaging was deployed to visualize 37 labeled antibodies at subcellular resolution in ductal carcinoma in situ (DCIS) revealing coordinated TME transitions to invasive breast cancer (IBC).
