Comprehensive Opal Multiplex Validation: From Staining to Digital Image Analysis
sponsored by Akoya Biosciences
January 13, 2021 | 4:00PM London, GMT
Optimisation and validation of a multiplex immunofluorescence workflow, from staining to digital image analysis, ensures assay robustness and prepares this technology for deployment in clinical trial analysis and in routine diagnostics. Chromogenic immunohistochemistry and fluorescent single-plexes are fundamental in this process, particularly when biomarkers are co-expressed. Amélie describes how she used the Opal Automation IHC kit and Vectra Polaris to develop two complementary multiplex immunofluorescence panels to evaluate the tumour immune microenvironment in colorectal cancer formalin-fixed paraffin-embedded tissue. During this talk, Amélie highlights the various parameters of staining, scanning and digital image analysis that should be considered when standardising a digital pathology workflow.
- Step-by-step process of how to optimise and validate staining and scanning protocols using the Opal Automation IHC kit and Vectra Polaris
- How to perform multi-marker analysis of multiplex immunofluorescence images in QuPath