Inside the Lab Digital and computational pathology, Technology and innovation, Microscopy and imaging, Oncology

Comprehensive Opal Multiplex Validation: From Staining to Digital Image Analysis

01/13/2021

January 13, 2021 | 4:00PM London, GMT

Optimisation and validation of a multiplex immunofluorescence workflow, from staining to digital image analysis, ensures assay robustness and prepares this technology for deployment in clinical trial analysis and in routine diagnostics. Chromogenic immunohistochemistry and fluorescent single-plexes are fundamental in this process, particularly when biomarkers are co-expressed. Amélie describes how she used the Opal Automation IHC kit and Vectra Polaris to develop two complementary multiplex immunofluorescence panels to evaluate the tumour immune microenvironment in colorectal cancer formalin-fixed paraffin-embedded tissue. During this talk, Amélie highlights the various parameters of staining, scanning and digital image analysis that should be considered when standardising a digital pathology workflow.

Learning Objectives:

Step-by-step process of how to optimise and validate staining and scanning protocols using the Opal Automation IHC kit and Vectra Polaris
How to perform multi-marker analysis of multiplex immunofluorescence images in QuPath

Available On-Demand

Visit our Webinar Hub

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

I want to stay up to date with the Retina field I want to stay up to date with the Infectious Diseases field I want to stay up to date with the Pathologist Educator field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.