Deep Blue Stain
A new stain offers beautiful possibilities in skin and gastrointestinal pathology
These images represent a new stain based on picro aniline, Ziehl’s fuchsin, and acetic acid. The stain is useful in dermatopathology because of its utility in differentiating squamous epithelium keratinization (yellow/brown) and lymphocytes (dark red). It is useful in gastric biopsies to differentiate metaplastic glands.
Protocol
Solution A: 100 mL distilled water + 1 drop Ziehl’s fuchsin (from Ziehl-Neelsen stain) + 1 drop 9% acetic acid
Solution B: 100 mL distilled water + 1 drop 10% formaldehyde + 1 drop 9% acetic acid
Solution C: Picro aniline
- Dewax and rehydrate.
- Wash 5–10 seconds in distilled water.
- Put in Solution A for 2–5 minutes.
- Wash 5–10 seconds in distilled water.
- Put in Solution B for 5 minutes.
- Wash 5–10 seconds in distilled water.
- Put picro aniline on sections for 5 minutes.
- Wash slides until blue color appears.
- Put in xylene for 3 minutes.
- Mount coverslips.
Submitted by Dmitry A. Zinovkin, pathologist at Gomel State Medical University, Gomel, Belarus.
Pathologist at Gomel State Medical University, Gomel, Belarus.
