MARQO is an open-source pipeline for cancer tissue analysis.
It enables single-cell resolution multiplex immunostaining analysis.
The method integrates image registration, segmentation, and classification.
Validation studies showed a Dice score of 83 percent in hepatocellular carcinoma.
The tool demonstrated correlation with manual pathologist quantification.
High computational capacity is required for operation.
MARQO is suitable for research but not yet routine diagnostics.
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