Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Pathologist
  • Explore Pathology

    Explore

    • Latest
    • Insights
    • Case Studies
    • Opinion & Personal Narratives
    • Research & Innovations
    • Product Profiles

    Featured Topics

    • Molecular Pathology
    • Infectious Disease
    • Digital Pathology

    Issues

    • Latest Issue
    • Archive
  • Subspecialties
    • Oncology
    • Histology
    • Cytology
    • Hematology
    • Endocrinology
    • Neurology
    • Microbiology & Immunology
    • Forensics
    • Pathologists' Assistants
  • Training & Education

    Career Development

    • Professional Development
    • Career Pathways
    • Workforce Trends

    Educational Resources

    • Guidelines & Recommendations
    • App Notes

    Events

    • Webinars
    • Live Events
  • Events
    • Live Events
    • Webinars
  • Profiles & Community

    People & Profiles

    • Power List
    • Voices in the Community
    • Authors & Contributors
  • Multimedia
    • Video
    • Podcasts
Subscribe
Subscribe

False

The Pathologist / Issues / 2020 / Sep / Illuminating Progress in Microscope Lighting
Microscopy and imaging Technology and innovation

Illuminating Progress in Microscope Lighting

Six reasons to try a LED light source for microscopy

By Klaus Willeke 09/16/2020 1 min read

Share

For pathologists, the color rendering of a microscope’s light source is critical for seeing stained samples correctly and making a reliable diagnosis. And that’s why many pathologists continue to use halogen lamps with filters – a method over 60 years old. The halogen bulb’s color rendering enables them to view colors in the vitally important pink, cyan, and purple ranges. But is there a better solution? Today’s modern light-emitting diode (LED) light sources can render colors with equal accuracy – and offer a few added benefits. In my view, there are six good reasons for making the switch…

Color rendering measures the ability of a light source to show a sample’s “true” colors – in other words, those that would be detected by the naked eye. This can be quantitatively measured on the color rendering index (CRI), a scale that runs from 0 to 100.

Halogen light sources with filters are known for their color rendering performance, with a CRI of 100. Although early LEDs couldn’t compete, modern LEDs score equal to or better than 96 – coming close to the performance of halogen lamps and enabling researchers to see true colors (see Figure 1).

Figure 1. A comparison between a halogen lamp with filter and a modern LED light source.

In this area, LEDs tend to get a bad rap – why? The light intensity of generic white LEDs can vary drastically at different wavelengths, peaking at 400–430 nm and weak from 600–700 nm. This variability can lead to a lack of intensity in the red end of the spectrum, causing a blue shift in the colors produced. A halogen lamp with a filter, on the other hand, provides smooth, continuous characteristics across the visible light spectrum, allowing uniform illumination of every color in a sample.

Unlike their earlier counterparts, modern LEDs designed for microscopy can closely match the spectral characteristics of halogen for accurate color representation (see Figure 2).

Figure 2. Spectral distributions of a halogen lamp with a filter (left, pink), a high-end LED (center, yellow), and a generic white LED (right, blue).

In the past, pathologists relied on halogen light sources with daylight filters to provide the right color temperature for viewing stained samples; white LEDs were known for their cooler color temperature, which altered the colors of common stains (see Figure 3).

Figure 3. Differences between a halogen lamp with filter (left) and LED illumination (middle). Adding a CC filter to the LED light source mitigates the issue (right), but does not fully correct it.

Today, the answer is not so black and white. Not only can modern LEDs match the color temperature of halogen lamps with filters, but they can overcome one of the difficulties of halogens: inconsistent color temperature at different brightness levels. Whereas halogen lamps’ color temperature changes with different voltages, LEDs provide a consistent color temperature at any brightness level. Notably, eye fatigue is reduced when eyes don’t have to adapt to changes in color.

When groups observe samples together, the images need to be clear and bright. Both halogen light sources and generic white LEDs lack the brightness required for large groups to view a sample simultaneously on a multi-headed microscope. Illuminators that use filters or fly-eye lenses face the risk of even lower luminosity. Fortunately, many modern LEDs are bright enough to allow multiple viewers to observe and discuss samples with no loss of image quality.

LEDs are known for their longevity. In general, modern LED light sources can last up to 50,000 hours – approximately 25 times the lifespan of a halogen bulb. A microscope used 40 hours a week with a 50,000-hour light source should last over 24 years. With a long-lasting LED light source, you may never need a replacement – saving money, helping the environment, and reducing time spent on microscope maintenance.

Halogen and generic LEDs sometimes require the user to adjust the brightness when changing magnification. If that happens often, it can reduce observation efficiency and cause eye strain. The good news is that modern LEDs remove this step entirely with automated light intensity – speeding up observations and improving pathologists’ comfort and eye health. LEDs also produce less heat than halogen bulbs – a benefit for pathologists who may spend hours or entire days at the microscope, working in small and easily overheated rooms.

In pathology’s “halogen versus LED” debate, halogen used to emerge victorious – but today, I believe LEDs have a clear edge. With reliable color reproduction, bright illumination, power efficiency, and user comfort, LED illumination’s popularity among pathologists is sure to continue growing.

Newsletters

Receive the latest pathology news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

About the Author(s)

Klaus Willeke

Product Marketing Manager for clinical and educational microscopes for Olympus Scientific Solutions’ Life Science division, Hamburg, Germany.

More Articles by Klaus Willeke

Explore More in Pathology

Dive deeper into the world of pathology. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Intense Intestines
Microscopy and imaging
Intense Intestines

January 19, 2024

1 min read

Some powerful fluorescent microscopy in this Image of the Month…

Context Matters in Cancer Biology
Microscopy and imaging
Context Matters in Cancer Biology

December 27, 2021

1 min read

Akoya is leading the way with spatial phenotypic signatures – a novel class of biomarkers for predicting response to immunotherapy

When Pathology Goes 3D
Microscopy and imaging
When Pathology Goes 3D

February 2, 2022

1 min read

Stratifying cancers with non-destructive 3D pathology

Sacrificing Safety for Speed?
Microscopy and imaging
Sacrificing Safety for Speed?

February 4, 2022

3 min read

Wide adaptation of antigen testing could increase the risk of exposure to infectious SARS-CoV-2 samples

False

The Pathologist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.