Cookies

Like most websites The Pathologist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.
Subscribe to Newsletter
Inside the Lab Hematology, Point of care testing, Laboratory management, Technology and innovation

Paper Versus Pancreatitis

As the pursuit to develop more sophisticated and sensitive diagnostics continues, the cost of producing those tools and techniques tends to increase, making them inaccessible to low-and middle-income countries. But moving forward doesn’t always have to come with a hefty price tag, as researchers from the Indian Institute of Science have shown with their new paper-based biosensor, which uses photoluminescence to detect lipase (1).

Why is this biomarker important? High levels of lipase in blood can indicate pancreatic inflammation. A quick, affordable diagnostic could aid in the preliminary diagnosis of pancreatitis, especially in low-and middle-income countries (LMICs) that may lack conventional diagnostic equipment – or even the electricity to power it.

The apparatus consists of a paper disc embedded in a terbium gel that contains a pro-sensitizer (a chemical that liberates the sensitizer). It costs approximately one and a half cents to produce five discs, making it an extremely cost-effective technique.

How exactly does the biosensor work? “Upon activation of the specific enzymes, the 2,3-dihydroxynaphthalene sensitizer is liberated, resulted in the ‘turning-on’ of green luminescence, detectible under UV light,” says Uday Maitra, study author and Professor in the Department of Organic Chemistry at the Indian Institute of Science. “The main idea with this is to chemically modify the sensitizer with an enzyme-cleavable group. The advantage, we felt, was that as long as we are able to design appropriate pro-sensitizers, all enzymes will be detected with the same fluorescence.”

Indeed, the investigators have big plans for the ultra-cheap biosensor. “At present, we have half a dozen pro-sensitizers, and we are developing more artificial substrates to detect more enzymes. On top of that, we’re improving the sensitivity, and fabricating a handheld device that can be used for imaging and quantifying the enzyme-triggered luminescence of the paper discs. So a lot of work remains to be done!”

Receive content, products, events as well as relevant industry updates from The Pathologist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

  1. T Gorai, U Maitra, “Supramolecular approach to enzyme sensing on paper discs using lanthanide photoluminescence”, ACS Sens, 1, 934-940 (2017).
Related Application Notes
ClearLLab 10C Panel Markers and how they are combined

| Contributed by Beckman Coulter Life Sciences

ClearLLab Control Cells - A Process Control for ClearLLab 10C Application

| Contributed by Beckman Coulter Life Sciences

The Analysis of FFPE Samples by Next-Generation Sequencing (NGS) of Key Genes for Research into Breast and Ovarian Cancer

| Contributed by Oxford Gene Technology

Most Popular
Register to The Pathologist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Pathologist magazine

Register