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Performance of NEXTFLEX Variant-Seq™ SARS-CoV-2 Kit using COVID-19 Positive Samples

Introduction

The NEXTFLEX Variant-Seq™ SARS-CoV-2 Kit identifies all mutations in a SARS-CoV-2 sample using Next Generation Sequencing (NGS). This is a powerful method to understand the full extent of diversity of variant SARS-CoV-2 viruses circulating in a population. The genomic data obtained provides information on multiple aspects that are relevant to understand and manage the global pandemic, such as early detection and characterization of emerging variants, virus transmission dynamics and the impact of response measures on the spread of the virus in a population.

One of the challenges of variant identification confidence is the variability in the Ct value of PCR-positive samples. The Ct value has been linked to the number of viral genome copies present in a sample, raising the question of how well the NGS workflow performs across different Ct values. We address this question in this note with data obtained using clinical samples.

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