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Inside the Lab Analytical science, Genetics and epigenetics, Omics

Label-free quantification of RNA methylations using targeted mass spectrometry

Epitranscriptomics - the study of RNA modifications - is an emerging and promising research field. RNA modifications are involved in all steps of post-transcriptional regulation of gene expression (RNA structure, splicing, export; stability, cellular localization and translation). Finally, theses modifications are essential for key cellular functions such as survival, growth and differentiation.

Here we describe an experimental workflow for the quantification of methylated nucleosides from cellular mRNA based on separation and detection on a LCMS-8060 system.

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