Identifying Collagen Fiber Types I and III Stained with Picrosirius Red Using the BX53 Microscope Equipped with Olympus’ High Luminosity and High Color Rendering LED

To observe collagen fibers, samples can be stained with Masson trichrome (MT), Elastica van Gieson (EVG), or Elastica Masson (E-M); however, distinction between collagen fiber types I and III is not possible with this method. In drug discovery research, quantitative evaluation of the collagen fibers is required, so it is necessary to be able to distinguish between collagen fiber typesⅠand III. Researchers in this field are turning their attention to polarized light observation of collagen fibers stained with Picrosirius Red because this method allows the required distinction between the two fiber types.