Development of a Multiplexed Panel for the Identification and Quality Control of Formalin- Fixed, Paraffin-Embedded Samples using the MassARRAY® System
contributed by Agena Bioscience |
Highly-annotated, well-sourced bio-specimens are essential in elucidating cancer initiation and progression and in validating panels for clinically actionable use. Formalin-fixed, paraffin-embedded (FFPE) tissue archives are most commonly used for oncology research and validation studies. However, accurate mutation detection is often problematic in FFPE tissue since it tends to have highly variable levels of fragmentation that limit the amount of usable DNA templates. In addition, formalin-introduced sequence artifacts and PCR inhibitors further obstruct mutation detection. The lack of consistency between the various protocols for sample handling and extraction of DNA is one of the major obstacles in translating analysis to clinical practice.
Read the full article now
Log in or register to read this article in full and gain access to The Pathologist’s entire content archive. It’s FREE and always will be!
Login if you already created an account
Or register now - it’s free and always will be!
You will benefit from:
- Unlimited access to ALL articles
- News, interviews & opinions from leading industry experts
- Receive print (and PDF) copies of The Pathologist magazine