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Development of a Multiplexed Panel for the Identification and Quality Control of Formalin- Fixed, Paraffin-Embedded Samples using the MassARRAY® System

Introduction

Highly-annotated, well-sourced bio-specimens are essential in elucidating cancer initiation and progression and in validating panels for clinically actionable use. Formalin-fixed, paraffin-embedded (FFPE) tissue archives are most commonly used for oncology research and validation studies. However, accurate mutation detection is often problematic in FFPE tissue since it tends to have highly variable levels of fragmentation that limit the amount of usable DNA templates. In addition, formalin-introduced sequence artifacts and PCR inhibitors further obstruct mutation detection. The lack of consistency between the various protocols for sample handling and extraction of DNA is one of the major obstacles in translating analysis to clinical practice.

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