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Comparison of Digital Image Analysis with Manual Reads for Chromogenic RNA ISH Slides

Abstract

RNA ISH (Ribonucleic acid in situ hybridization) assays are an ever-expanding application due to the ability to evaluate molecular targets, while retaining tissue morphology. The rate limiting step in RNA ISH assays is the time consuming and error prone method of manually counting signal under a microscope. The Aperio RNA ISH Algorithm offers a reproducible, fast, and quantitative method of evaluating tissue samples that have been stained to detect RNA ISH signal. This single algorithm can be used on numerous tissue types for both single and dual-plex assays.

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