Cookies

Like most websites The Pathologist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Assessment of the performance of a hybridisation-based NGS enrichment panel with as little as 10 ng of severely formalin-compromised DNA

Jacqueline Chan, Juliette Forster, Aysel Heckel, Venu Pullabhatla, Dave Cook, Graham Speight
Oxford Gene Technology (OGT)

Introduction

Formalin-fixed, paraffin-embedded (FFPE) storage is a standard method for archiving samples from solid tumours. It ensures the preservation of the ultrastructure of tissues and prevents degradation through formation of chemical links between macromolecules, for example between and within DNA molecules. FFPE samples contain a wealth of information which can be used to study cancer development and progression. Next generation sequencing (NGS) offers the capability of unlocking this information through the simultaneous study of multiple types of mutations in cancer-associated genes for a number of applications1. However, formalin treatment can significantly compromise the quality and amount of nucleic acids available for genomics research. As such it is technically challenging to examine the true genetic complexity present in a sample.

    Read the full article now

    Log in or register to read this article in full and gain access to The Pathologist’s entire content archive. It’s FREE!

    Login
    Receive content, products, events as well as relevant industry updates from The Pathologist and its sponsors.

    When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
    If you wish to unsubscribe, you can update your preferences at any point.

    Register to The Pathologist

    Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

    You will benefit from:
    • Unlimited access to ALL articles
    • News, interviews & opinions from leading industry experts
    • Receive print (and PDF) copies of The Pathologist magazine

    Register