This application describes the analysis of one corticosteroid the Dexamethasone and 3 antibiotics the Tazobactam, Meropenem and Piperacillin. Dexamethasone is a synthetic glucocorticoid hormone. It has an anti-inflammatory and immunosuppressive effect. It is widely used for reducing swelling associated with tumors, and treat eye inflammation. Dexamethasone can help treat allergic reactions and be used in cancer treatments too1.
More recently, studies have demonstrated the effectiveness of dexamethasone in the treatment of COVID-19, mainly for person with acute respiratory distress syndrome (ARDS). Corticosteroid therapy reduced the risk of all-cause mortality (risk ratio 0.75; 95% CI, 0.59–0.95)2. In order to avoid the risk of bacterial overgrowth, which is more common with corticosteroid treatment, an antibiotic therapy is often combined with it2.
This following methods allows the simultaneous quantification of corticoid and antibiotic in biological samples with a fully automated sample preparation due to the use of CLAM system.
By simplifying uncapping blood collection tubes (or sample cups), placing the pretreatment vials in the system, and requesting analysis, the system performs all other process steps automatically, from pretreatment to LCMS analysis.
This fast analytical method (=5min) and its automatic and overlapped sample preparation allows to treat a large number of samples in a minimum of time. The sensitivity of the LCMS-8060 allows to sweep samples with an analysis dynamic range from 1 to 100 ng/mL.
>> Download the Application Note as a PDF
Newsletters
Receive the latest pathology news, personalities, education, and career development – weekly to your inbox.
