To develop a robust, quick, and reliable method using the Thermo Scientific™ TSQ Altis™ MD mass spectrometer to allow the absolute quantitation of peptides from SARS-CoV-2 enzymatic digestions.
- Simple offline sample preparation, with high recovery
- Reliable detection and absolute quantification of peptides from digested SARS-CoV-2 spike and nucleocapsid proteins
- down to low/sub-femtomole on-column concentrations
- Highlights the viability of two different sample matrices
The development of a bottom-up proteolytic workflow has been demonstrated as a reliable means of detection for SARS-CoV-2 peptides that are biomarkers of an active COVID-19 infection.1 In this technical note, a quick and robust mass spectrometry-based method for the detection of such biomarkers is reported. Six peptides were observed from recombinant protein samples that were spiked either onto nasopharyngeal swabs or into saliva, placed in viral transport media, and enzymatically digested. Sub/low-femtomole on-column detection and quantification limits were observed for each peptide, demonstrating the feasibility of using a TSQ Altis MD mass spectrometer for COVID-19 testing.
>> Download the Application Note as a PDF
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