This white paper is to introduce ClearLLab Control Cells. Flow Cytometry immunophenotyping is a commonly used technology as an aid in diagnosis of hematolymphoid malignancies. ClearLLab 10C Panels are composed of 4 independent immunophenotyping reagents including 27 unique makers which are consistent with WHO and Bethesda guidelines. To be compliant, the performance of reagents and staining procedures needs to be verified using positive control materials. ClearLLab Control Cells were developed to support ClearLLab 10C Panels and include Normal Control and Abnormal Control Cells. ClearLLab Control Cells are whole blood based products that can consistently provide process/positive controls for all the markers/antigens in ClearLLab 10C Panels.
Multiple lots of both fresh (*T0M) and aged (*T3M+Op-1M) Control cells were stained with each of the four ClearLLab 10C Panels to assess the staining signal, resolution of each antigen as well as specimen stability. In addition, unique gating strategies have been developed for Control Cell data acquisition and analysis to provide an easy, simple and consistent assessment for each relevant cell population.
Normal or Abnormal Control Cells
- Pre-wash control cells three times with PBS+2% FBS (0.45mL:12mL)
- Stain washed cells (100μL) with ClearLLab 10C T, B, M1 or M2 Cell Tubes
- Lyse cells with IOTest3 Lysing Plus Fixative Solution (2mL per sample)
- Spin and aspirate, Wash again with 1x PBS (3mL per sample)
- Spin and aspirate, suspend cell pellet with 1x PBS (0.5mL per sample)
- Acquire sample with Navios or Navios EX Cytometer Instrument
- Analyze Data with Kaluza C Software version 1.5
>> Download the Full Application Note as a PDF
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