Flow cytometric immunophenotyping remains an indispensable tool for the diagnosis, classification, staging, and monitoring of hematologic neoplasms. There have been significant advances in flow cytometry instrumentation and availability of an expanded range of antibodies and fluorochromes that have improved the ability to identify different normal cell populations and recognize phenotypic aberrancies.
ClearLLab 10C Panels consists of a set of four 10-Color reagents in dry unitized format, composed of antibodies directed against T, B, NK and Myeloid lineage antigens. These panels are designed to include the primary antibodies which are lineage oriented as per the 2006 Bethesda consensus. The combinations are intended to identify the leukocyte subpopulations that express these antigens alone or in specific co-expression patterns, and are designed to work with both normal samples and samples coming from any patient having or suspected of having the following hematopoietic neoplasms: chronic leukemia, acute leukemia, non-Hodgkin lymphoma, myeloma, myelodysplastic syndrome (MDS), and/or myeloproliferative neoplasms (MPN). The specific choices and combinations in the ClearLLab 10C Panels are based on the guiding principles of (1) addressing the clinical indications, (2) accounting for all major cell populations present in the specimen, and (3) providing sufficiently comprehensive identification of all major categories of hematopoietic cell populations in both normal and neoplastic states. (1-10) The normal cells within the sample act as an internal reference and can be used to determine whether a marker is brighter or dimmer than expected on a specific cell population. The inclusion of a backbone and redundant markers within the panels makes it easier to track populations between tubes, merge the data and troubleshoot unexpected results. In the panel the backbone of CD45 and CD34 are included in each tube. CD45 is used for consistent gating across all the four tubes. CD34 in combination with weak CD45 positivity can be used to confirm the hematopoietic origin of cells. Other redundant markers have been included in the panels such as CD13 and HLA-DR, in both M1 and M2 tubes.
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