Formalin-fixed paraffin-embedded (FFPE) tissue samples are a standard clinical sample type that serve as an invaluable repository of information for oncology research. With recent advances in next-generation sequencing (NGS) technology, genomic information preserved in these samples can now be recovered to yield important insights.
Targeted NGS is increasingly used to assess the genomic alterations in oncology. While there are several commercially available amplicon-based target enrichment technologies, not all are created equal.
In this technical note, the expert team at SOPHiA GENETICS compared the performance of Paragon Genomics’ CleanPlex® OncoZoom® Cancer Hotspot Panel to three other commercially available amplicon-based cancer hotspot panels using DNA of varying quality and quantity extracted from FFPE tissue samples.
The CleanPlex OncoZoom Cancer Hotspot Panel allows for rapid detection and characterization of somatic mutations across the hotspot regions of 65 oncogenes and tumor suppressor genes. The panel is powered by Paragon Genomics' CleanPlex technology which utilizes a proprietary background cleaning chemistry to remove nonspecific PCR products to produce NGS libraries with the highest coverage uniformity.
The combination of CleanPlex and SOPHiA™ AI provides a rapid and complete solution from sample to highly reliable genomic data analysis, interpretation, and reporting.
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