Cellular localization of RNA expression in central and peripheral nervous system using RNAscope® Technology

Detect your RNA of interest in the central and peripheral nervous system with ease

• Detection of virtually any gene in any genome in any tissue with high sensitivity and specificity
• Visualize RNA expression and distribution with morphological context
• Uncover lncRNAs and other targets for which antibodies cannot detect

Detection of RNA in nervous system using RNAscope® Technology

Neuroscience is one of the fastest growing research fields, studying the structural and functional organization and the development of the central and peripheral nervous system from the molecular and cellular level to the systems level.

One challenge in the neuroscience field is the numerous cell types in the central nervous system (CNS), many of which remain to be identified and characterized at the molecular level.

RNAscope^® in situ hybridization (ISH) technology enables cell-specific localization of RNA transcripts precisely in the tissue to identify markers associated with neuronal and synaptic molecular pathways and to characterize specific cell types such as neuronal and glial cells.

The RNAscope^® assay can be used to:

• Identify, characterize, and localize mRNAs in the central and peripheral nervous systems
• Detect mRNA expression in axons and dendrites
• Detect mRNA in the nervous system when no reliable antibodies are available
• Identify long non-coding RNA in neuronal and glial cells
• Detect mRNA in primary neuronal cell cultures

RNAscope^® ISH technology provides a unique detection method for neuronal and glial cells markers that is both highly sensitive and specific for detection of any gene expressed in any neuronal and glial cell¹. Because of its proprietary “double Z” probe, RNAscope^® technology is a powerful tool to detect any gene, especially low abundance targets. And not only can RNAscope^® technology be used to discern if your gene of interest is in a neuronal or glial cell, but it can also show its subcellular localization. The multiplexing capabilities of RNAscope^® assay allows for detection of multiple markers at once, enabling exquisite characterization of cell populations within the nervous system as well as detection of both neuronal and glial cell markers and the signals these cells produce. RNAscope® technology also helps solve the problem when no reliable antibodies exist for your gene of interest and allows you to visualize and quantify for virtually any gene from any genome in any tissue.

FIGURE 1. Detection of Otx2 mRNA in mouse cerebellum FFPE tissue with the RNAscope® 2.5 HD Reagent Kit - BROWN.

FIGURE 2. Mash1 mRNA expression in mouse brain tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 HD Reagent Kit - BROWN.

>> Download the full Application Note as PDF

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