Introduction
Understanding molecular mechanisms is critical for identifying pathogenesis, evaluating disease progression and developing new therapeutic strategies for autoimmunity, infectious diseases, and cancer. RNAscope® in situ hybridization (ISH) technology enables precise cell- and tissue-specific localization of RNA transcripts. With RNAscope® ISH assays, you can detect specifically expressed immune markers in lymphoid and non-lymphoid tissues while maintaining the morphological context. For example, RNAscope® chromogenic in situ hybridization (CISH) robustly identified the expression pattern of CD274 mRNA in formalin-fixed paraffin-embedded (FFPE) kidney cancer tissue (Figure 1).


